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STRUCTURAL & FUNCTIONAL BIOCHEMISTRY

 
Selections
1. Brief Description 6. Thesis
2. Research Interests 7. Photos
3. Personnel 8. Useful links
4. Funded Research Projects 9. Contact
5. Publications    

1. Brief Description:
The Structural and Functional Biochemistry Laboratory serves the educational and research needs in the disciplines of biochemistry (intermediate metabolism, synthesis and nucleic acid metabolism, synthesis, structure and function of proteins), structural biochemistry, proteomics, structural genomics, regulation and Metabolic Control of Enzymes and the implementation of all the above in Analytical Biochemistry, Enzymology Clinical Biochemistry, Food biochemistry, Chemical Biology, structure-driven drug design and Biophysics as applied to Biochemical Sciences.
 
2. Research interests:
The general theme that underlies the work of the SFBG is macromolecular structural and functional biochemistry in which protein structures and protein interactions within complexes in solution are used as the starting point for further studies on the mechanisms of catalysis, mechanisms of inhibition, regulation, molecular recognition, and structure-based drug design.
Currently our activities are directed to research on structural and functional aspects of macromolecules of pharmaceutical and biotechnological interest and structure-based drug design. The main directions of the group's research activities is the rational design, synthesis, and evaluation of molecules against major diseases with a current emphasis on type 2 diabetes, cancer, inflammation, and thrombosis. Research in SFBG also aims to elucidate the correlation between structure/function of enzymes involved in glycogen metabolism, ribonucleases of the pancreatic ribonuclease A superfamily, deadenylases which are the enzymes that degrade poly(A) tails regulating mRNA turnover rates mitochondrial steroid hormone receptors, and gene expression and homeostasis factors. Specific project within the laboratory are as
follows:
 
3. Personnel:
  • Mr. Georgios Stravodimos, PhD. Student
  • Mr. Athanasios Kiritsis, PhD. Student
  • Mr. Viron Georgogietas, PhD. Student
  • Ms Aikaterin Karra, PhD. Student
  • Ms Theodora Solovou, PhD. Student
  • Ms Ioanna Theochari, PhD. Student
  • Ms Panagiota Liggri, PhD. Student
  • Ms Myrsini Michou, PhD. Student
  • Ms Rafailia Beta, PhD. Student
  • Mr Eftymios Kyriakis, PhD. Student
  • Mr  Georgios Moros, PhD. Student
  • Ms Olga Papaioannou, PhD. Student
  • Mr Dionysios Antonopoulos, PhD. Student
  • Ms Athina Keramidiotou, PhD. Student
  • Mr Konstantinos Tsilafakis, PhD. Student
  • Mr Ionnis Tsialtas, PhD. Student
  • Ms Aikaterini Karagiorgou, PhD. Student

4. Funded Research projects:

  • University of Thessaly Research Committee. “Proteomic analysis of the entomopathogeny of Pseudomonas entomophila”. Grant No. 4098. PI: Μ.Κοntou
  • University of Thessaly Research Committee. «Studies on function and structure of poly(A) specific ribonuclease, PARN». Grant No. 3743. PI: Nikolaos Balatsos.
  • Ministry of National Education and Religion Affairs Project Eraklitos IΙ: «Τranscriptomic and proteomic analysis of the most important parasite of the olive, the insect Bactrocera oleae, with emphasis on systems gender segregation and resistance to insecticides». M.Κοntou, collaborator
  • University of Thessaly Research Committee. "Structure and function studies of the poly(A) specific ribonuclease PARN". Grant No.3743. PI: Nikolaos Balatsos.
  • University of Thessaly Research Committee. "Role of steroid hormone receptors in mitochondria functions” Grant No. 4088.14.01. PI: Α.-Μ. Psarra.
  • General Secretariat for Research and Technology, Joint Research Projects Greece - France (2010-2012): Human angiogenin as a molecular target for the development of new anticancer agents. PI: D.D. Leonidas
  • Structure-assisted Design, Synthesis, and Evaluation of Bioactive compounds for type 2 Diabetes mellitus" "ARISTEIA" Action of the "OPERATIONAL PROGRAMME EDUCATION AND LIFELONG LEARNING" and is co-funded by the European Social Fund (ESF) and National Resources.

5. Publications (2005 - ):
  1. Structural analysis of the Rhizoctonia solani agglutinin reveals a domain-swapping dimeric assembly.Skamnaki, V.T., Peumans, W.J., Kantsadi, A.L., Cubeta, M.A., Plas, K., Pakala, S., Zographos, S.E., Smagghe, G., Nierman, W.C., Van Damme, E.J., Leonidas, D.D. (2013) FEBS J. 280, 1750-1763.
  2. Sourcing the affinity of flavonoids for the glycogen phosphorylase inhibitor site via crystallography, kinetics and QM/MM-PBSA binding studies: Comparison of chrysin and flavopiridol.Tsitsanou, K.E., Hayes, J.M., Keramioti, M., Mamais, M., Oikonomakos, N.G., Kato, A., Leonidas, D.D., Zographos, S.E. (2013) Food Chem Toxicol.,in press
  3. Studies on the essential intramolecular interaction between the A1 and A2 domains of von Willebrand factor. Karoulia, Z., Papadopoulos, G., Nomikos, M., Thanassoulas, A., Choli-Papadopoulou, T., Nounessis, G., Kontou, M., Stathopoulos, C., and Leonidas, D.D. (2013). Prot. & Pept. Lett. 20, 231-240.
  4. Triazole pyrimidine nucleosides as inhibitors of Ribonuclease A. Synthesis, biochemical, and structural evaluation. Parmenopoulou, V., Chatzileontiadou, D.S.M., Manta, S., Bougiatioti, S., Maragozidis, P., Gkaragkouni,D.-N., Kaffesaki, E., Kantsadi, A.L., Skamnaki, V.T., Zographos, S.E., Zoumpoulakis, P., Balatsos, N.A.A., Komiotis, D., and Leonidas, D.D. (2012). Bioorg Med Chem. 20, 7184-7193
  5. The binding of C5-alkynyl and alkylfurano[2,3-d]pyrimidine glucopyranonucleosides to glycogen phosphorylase b. Synthesis, biochemical and biological assessment. Kantsadi, A.L., Manta, S., Psarra, A.-M.G., Dimopoulou, A., Kiritsis, C., Parmenopoulou, V., Skamnaki, V.T., Zoumpoulakis, P., Zographos, S.E., Leonidas, D.D., and Komiotis D. (2012) Eur. J. Med. Chem. 54, 740-749.
  6. The σ-Hole Phenomenon of Halogen Atoms Forms the Structural Basis of the Strong Inhibitory Potency of C5 Halogen Substituted Glucopyranosyl Nucleosides towards Glycogen Phosphorylase b. Kantsadi AL, Hayes JM, Manta S, Skamnaki VT, Kiritsis C, Psarra AM, Koutsogiannis Z, Dimopoulou A, Theofanous S, Nikoleousakos N, Zoumpoulakis P, Kontou M, Papadopoulos G, Zographos SE, Komiotis D, Leonidas DD.(2012) ChemMedChem 7,722-32
  7. 3'-axial CH(2) OH substitution on glucopyranose does not increase glycogen phosphorylase inhibitory potency. QM/MM-PBSA calculations suggest why. Manta S, Xipnitou A, Kiritsis C, Kantsadi AL, Hayes JM, Skamnaki VT, Lamprakis C, Kontou M, Zoumpoulakis P, Zographos SE,Leonidas DD, Komiotis D. (2012) Chem Biol Drug Des.79,663-73
  8. Kinetic and in silico analysis of the slow-binding inhibition of human poly(A)-specific ribonuclease (PARN) by novel nucleoside analogues. Balatsos N, Vlachakis D, Chatzigeorgiou V, Manta S, Komiotis D, Vlassi M, Stathopoulos C. (2012) Biochimie. 94, 214-221.
  9. Anopheles gambiae odorant binding protein crystal complex with the synthetic repellent DEET: implications for structure-based design of novel mosquito repellents. Tsitsanou KE, Thireou T, Drakou CE, Koussis K, Keramioti MV, Leonidas DD, Eliopoulos E, Iatrou K, Zographos SE.(2012) Cell Mol Life Sci. 69, 283-297.
  10. hCINAP is an atypical mammalian nuclear adenylate kinase with an ATPase motif: structural and functional studies. Drakou CE, Malekkou A, Hayes JM, Lederer CW, Leonidas DD, Oikonomakos NG, Lamond AI, Santama N, Zographos SE. (2012) Proteins 80, 206-220.
  11. Halogen-substituted (C-β-D-glucopyranosyl)-hydroquinone regioisomers: synthesis, enzymatic evaluation and their binding to glycogen phosphorylase. Alexacou KM, Zhang YZ, Praly JP, Zographos SE, Chrysina ED, Oikonomakos NG, Leonidas DD. (2011) Bioorg Med Chem. 19, 5125-5136.
  12. Computation as a tool for glycogen phosphorylase inhibitor design. Hayes, J.M., and Leonidas, D.D. (2010). Mini Rev Med Chem. 10. 1156-1174.
  13. The binding of β-D-glucopyranosyl-thiosemicarbazone derivatives to glycogen phosphorylase: a new class of inhibitors. Alexacou, K.-M., Tenchiu (Deleanu), A.-C., Chrysina, E.D., Charavgi, M.-D., Kostas, I.D., Zographos, S.E., Oikonomakos, N.G., and Leonidas, D.D. (2010).Biorg. Med. Chem. 18, 7911-7922
  14. Simple method for coating Si (100) surfaces with Ferritin monolayers - Iron Oxide Quantum Dots. Papadopoulos, G., Anetakis, C., Gravalidis, C., Kassavetis, S., Vouroutzis, N., Frangis, N., and Logothetidis, S. (2010).Mat Sci Eng., in press
  15. A Shape Descriptor for Fast Complementarity Matching in Molecular Docking. Axenopoulos, A., Daras, P., Papadopoulos, G., and Houstis, E. (2010). Trans. Comp. Biol. Bionf., in press.
  16. 1-(3-Deoxy-3-fluoro-beta-d-glucopyranosyl) pyrimidine derivatives as inhibitors of glycogen phosphorylase b: Kinetic, crystallographic and modelling studies. Tsirkone VG, Tsoukala E, Lamprakis C, Manta S, Hayes JM, Skamnaki VT, Drakou C, Zographos SE, Komiotis D, Leonidas DD (2010) Biorg. Med. Chem. 18, 3413-3425
  17. Glucose-based spiro-isoxazolines: a new family of potent glycogen phosphorylase inhibitors.Benltifa M, Hayes JM, Vidal S, Gueyrard D, Goekjian PG, Praly JP, Kizilis G, Tiraidis C, Alexacou KM, Chrysina ED, Zographos SE, Leonidas DD, Archontis G, Oikonomakos NG.(2009) Biorg. Med. Chem. 17,7368-7380.
  18. Mapping the ribonucleolytic active site of bovine seminal ribonuclease. The binding of pyrimidinyl phosphonucleotide inhibitors. K Dossi, V.G. Tsirkone, J.M. Hayes, J. Matoušek, P. Poučková, J. Souček, M. Zadinova, S.E. Zographos, and D.D. Leonidas (2009) Eur. J. Med. Chem. 44, 4496-4508
  19. Inhibitor design to Ribonuclease A: The binding of two 5´phosphate uridine analogues V.G. Tsirkone, K. Dossi, C. Drakou, S.E. Zographos, M. Kontou and D.D. Leonidas (2009) Acta Crystallogr. F65, 671-677. Cover Story
  20. Amide-1,2,3-triazole bioisosterism: the glycogen phosphorylase case. E.D. Chrysina, É. Bokor; K.-M. Alexacou, M.-D. Charavgi, G.N. Oikonomakos, S. E. Zographos; D.D. Leonidas, N.G. Oikonomakos, and L. Somsák (2009) Tetrahedron Asymm. 20, 733-740.
  21. Influence of Naturally-occurring 5′-Pyrophosphate-linked Substituents on the Binding of Adenylic Inhibitors to Ribonuclease A: an X-Ray Crystallographic Study. Holloway, D.E., Chavali, G.B., Leonidas, D.D., Baker, M.D., and Acharya, K.R. (2009). Biopolymers 91, 995-1008.
  22. Morpholino, piperidino, and pyrrolidino derivatives of pyrimidine nucleosides as inhibitors of Ribonuclease A: synthesis, biochemical and crystallographic evaluation. Samanta, A., Leonidas, D.D., Dasgupta, S., Pathak, T., Zographos, S.E., and Oikonomakos, N.G. (2009). J. Med. Chem. 52, 932-942
  23. Competitive inhibition of human poly(A)-specific ribonuclease (PARN) by synthetic fluoro-pyranosyl nucleosides. Balatsos N.A.A., Vlachakis D., Maragozidis P., Manta S., Anastasakis D., Kyritsis A., Vlassi M, Komiotis D. and Stathopoulos C. (2009). Biochemistry 48, 6044-6051.
  24. Inhibition of human poly(A) specific ribonucleases by purine nucleotides: kinetic analysis. Balatsos N.A.A., Anastasakis D. and Stathopoulos C. (2009). J Enzyme Inhib Med Chem. 24, 516-523.
  25. Glucocorticoid receptors and other nuclear transcription factors in mitochondria and possible functions. Psarra, A.-M.G, and Sekeris C.E. (2009) Biochim Biophys. Acta. 1787, 431-436.
  26. Interaction of mitochondrial thioredoxin with glucocorticoid receptor and NF-kappaB modulates glucocorticoid receptor and NF-kappaB signalling in HEK-293 cells. Psarra, A.-M.G, Hermann, S, Panayotou, G, Spyrou G. (2009) Biochem J. 422, 521-531.
  27. The C-terminal Region of HPNAP Activates Neutrophils and Promotes Their Adhesion to Endothelial Cells. Kottakis, F., Befani, C., Asiminas, A., Kontou, M., Koliakos, G., and Choli-Papadopoulou, T. (2009) Helicobacter 14, 177-179.
  28. Steroid and Thyroid Hormone Receptors in Mitochondria. Psarra, A.-M.G, and Sekeris, C.E. (2008) IUBMB Life 60, 210-23.
  29. New inhibitors of Glycogen Phosphorylase as potential Antidiabetic Agents. Somsak, L., Czifrak, K., Toth, M., Bokor, E., Chrysina, E.D., Alexacou, K.-M., Hayes, J.M., Tiraidis, C., Lazoura, E., Leonidas, D.D., Zographos, S.E., and Oikonomakos, N.G. (2008). Curr. Med. Chem. 15, 2933-2983.
  30. Pentacyclic triterpenes, inhibitors of glycogen phosphorylase, as potential drugs for type 2 diabetes: X-ray crystallographic studies. Zographos, S.E., Leonidas, D.D., Alexacou, K.M., Gimisis, T., Hayes, J.M., Oikonomakos, N.G, Wen, X., Sun, H., Liu, J., Cheng, K., Zhang, P., Zhang, L., Hao, J., Zhang, L., and Ni, P. (2008).Planta Medica 74, 1146-1147.
  31. Naturally Occurring Pentacyclic Triterpenes as Inhibitors of Glycogen Phosphorylase: Synthesis, Structure-Activity Relationships and X-ray Crystallographic Studies. X. Wen, H. Sun, J. Liu, K. Cheng, P. Zhang, L. Zhang, J. Hao, L. Zhang, P. Ni, S.E. Zographos, D. D. Leonidas,K.-M. Alexacou, T. Gimisis, J.M. Hayes, and N.G. Oikonomakos (2008). J. Med. Chem. 51, 3540-3554
  32. X ray sequence ambiguities of Sclerotium rolfsii lectin resolved by mass spectrometry. G.J. Sathisha, Y.K. Subrahmanya Prakash, V.B. Chachadi, N.N. Nagaraja, S.R.Inamdar, D.D. Leonidas, H.S. Savithri, and B.M. Swamy (2008). Amino Acids 5, 309-320
  33. Crystallographic and Computational studies on 4-phenyl-N-(β-D-glucopyranosyl)-1H-1,2,3-triazole-1-acetamide, an inhibitor of glycogen phosphorylase: comparison with a-D-glucose, N-acetyl-b-D-glucopyranosylamine and N-benzoyl-N´-b-D-glucopyranosyl urea binding. K.-M. Alexacou, J.M. Hayes, C. Tiraidis, S.E. Zographos, D.D. Leonidas, E.D. Chrysina, G. Archontis, N.G. Oikonomakos, J.V. Paul, B. Varghese, and D. Loganathan (2008). Proteins 71, 1307-1323.
  34. Synthesis and Evaluation of Functional Hyperbranched Polyether Polyols as Prospected Gene Carriers. Tziveleka, L.-A., Psarra, A.-M.G., Tsiourvas, D., and Paleos, C.M. (2008) Int. J. Pharm. 356, 314-324.
  35. Helicobacter pylori neutrophil-activating protein activates neutrophils by its C-terminal region even without dodecamer formation, which is a prerequisite for DNA protection - Novel approaches against Helicobacter pylori inflammation. Kottakis, F., Papadopoulos, G., Pappa, E.V., Cordopatis, P., Pentas, S., Choli-Papadopoulou, T. (2008). FEBS J. 275, 302-317
  36. FR25900, a potential anti-hyperglycemic drug, binds at the allosteric site of glycogen phosphorylase. C. Tiraidis, K.-M. Alexacou, S.E. Zogrpahos, D.D. Leonidas, T. Gimisis, and N.G. Oikonomakos (2007). Protein Sci 16, 1773-1782.
  37. Structural basis of the carbohydrate recognition of the Sclerotium rolfsii lectin. D.D. Leonidas, B.M. Swamy, G.N. Hatzopoulos, S.J. Gonchigar, V.B. Chachadi, S.R. Inamdar, S.E. Zographos, and N.G. Oikonomakos (2007). J. Mol. Biol. 368, 1145-1161
  38. In the Search of Glycogen Phosphorylase Inhibitors: Synthesis of C-D-Glycopyranosyl-benzo(hydro) quinones. Inhibition of and binding to glycogen phosphorylase in the crystal. L. He, Y.Z. Zang, M. Tanoh, G.-R. Chen, J.-P. Praly, E.D. Chrysina, M.N. Kosmopoulou, D.D. Leonidas, and N.G. Oikonomakos (2007). Eur. J. Org. Chem. 596-606.
  39. Recognition of Ribonuclease A by 3 '-5'-Pyrophosphate-linked Dinucleotide Inhibitors: a Molecular Dynamics/Continuum Electrostatics Analysis. S. Polydoridis, D.D. Leonidas, N.G. Oikonomakos, and G. Archontis. (2007). Biophys. J. 92, 1659-1672
  40. A multifunctional RNA recognition motif in poly(A)-specific ribonuclease with cap and poly(A) binding properties. Nilsson, P., Henriksson, N., Niedzwiecka A., Balatsos, N.A.A., Kokkoris K, Eriksson J. and Virtanen, A. (2007). J Biol Chem. 282, 32902-32911.
  41. The contribution of the zinc-finger motif to the function of Thermus thermophilus ribosomal protein S14. Xaplanteri, M.A., Papadopoulos, G., Leontiadou, F., Choli-Papadopoulou, T., and Kalpaxis, D. L. (2007). J.Mol.Biol. 369, 489-497
  42. Molecular cloning and biochemical characterization of VIM-12, a novel hybrid VIM-1/VIM-2 metallo-beta-lactamase from a Klebsiella pneumoniae clinical isolate, reveal atypical substrate specificity. Kontou, M., Pournaras, S., Kristo, I., Ikonomidis, A., Maniatis, A.N., and Stathopoulos, C. (2007) Biochemistry 46, 13170-13178.
  43. Prevalence of sleep-related symptoms in a primary care population - Their relation to asthma and COPD. Karachaliou F, Kostikas K, Pastaka C, Bagiatis V, Gourgoulianis K.I (2007) Prim Care Resp J 16, 222-228.
  44. Socioeconomic interactions of fishries and aquqculture in Greece: A case study of South Evoikos Gulf. Mente E, Pantazis P, Neofitou C, Aifanti S, Santos M.B, Bagiatis V, Papanagiotou E, Kourkouta V, Soutsas K. (2007) Aquaculture Economics and Management 11 (3), pp. 313-334.
  45. Regulation of hepatic glycogen metabolism by purine nucleoside site inhibitors of glycogen phosphorylase. Hampson, L.J., Arden, C., Agius, L., Ganotidis, M., Kosmopoulou, M.N., Tiraidis, C., Elemes, Y., Sakarellos, C., Leonidas, D.D., and Oikonomakos, N.G. (2006). Bioorg. Med. Chem. 14, 7835-7845
  46. Iminosugars as potential inhibitors of glycogenolysis: structural insights into the molecular basis of glycogen phosphorylase inhibition. Oikonomakos, N.G., Tiraidis, C., Leonidas, D.D., Zographos, S.E., Kristiansen, M., Jessen, C.U., Nørskov-Lauritsen, L., and Agius, L. (2006). J. Med. Chem. 49, 5687-5701.
  47. The binding of 3´-N-piperidine-4-carboxyl-3´-deoxy-ara-uridine to Ribonuclease A in the crystal. D.D. Leonidas, T.K. Maiti, A. Samanta, S. Dasgupta, T. Pathak, S.E. Zographos, and N.G. Oikonomakos (2006). Bioorg. Med. Chem. 14, 6055-6064.
  48. Indirubin and indigo analogues as potential inhibitors of glycogenolysis: structural basis of glycogen phosphorylase inhibition. N. G. Oikonomakos, M. N. Kosmopoulou, D. D. Leonidas, E. D. Chrysina, C. Tiraidis, N. Bischler, K. E. Tsitsanou, S. E. Zographos, I. D. Kostas, and G. Eisenbrand (2006).Indirubin, the red shade of indigo (Meijer L., Guyard N., Skaltsounis L. & Eisenbrand G., eds) 18, 177-189.
  49. Crystallographic studies on N-azidoacetyl-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine. E.I. Petsalakis, E.D. Chrysina, C. Tiraidis, T. Hadjiloi, D.D. Leonidas, N.G. Oikonomakos, U. Aich, B. Varghese, and D. Loganathan (2006).Bioorg. Med. Chem. 14, 5316-5324.
  50. Binding of oxalyl derivatives of beta-d-glucopyranosylamine to muscle glycogen phosphorylase b. T. Hadjiloi, C. Tiraidis, E.D. Chrysina, D.D. Leonidas, N.G. Oikonomakos, P. Tsipos, and T. Gimisis. (2006)Biorg. Med. Chem. 14, 3872-3882. Cover Story
  51. Crystallographic studies on two bioisosteric analogues, N-acetyl-beta-d-glucopyranosylamine and N-trifluoroacetyl-beta-d-glucopyranosylamine, potent inhibitors of muscle glycogen phosphorylase. E. Anagnostou, M.N. Kosmopoulou, E.D. Chrysina, D.D. Leonidas, T. Hatdjiloi, C. Tiraidis, S.E. Zographos, Z. Gyorgydeak, L. Somsak, T. Dosca, P. Gergely, F.N. Kolisis, and N.G. Oikonomakos (2006).Bioorg. Med. Chem. 14, 181-189.Cover Story
  52. Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC). Balatsos, N.A.A., Nilsson, P., Mazza, C., Cusack, S. and Virtanen, A. (2006). J. Biol. Chem. 281, 4517-4522.
  53. Comparison of the blood redox status between long-distance and short-distance runner. Kostaropoulos, I.A., Nikolaidis, M.G., Jamurtas, A.Z., Ikonomou, G.V., Makrygiannis, V., Papadopoulos. G., Kouretas, D. (2006). Physiol Res. 55, 611-616.
  54. Changes in the rate of poly(Phe) synthesis in E. coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: A Molecular Dynamics Simulation analysis. Papadopoulos, G., Grudinin, S., Kalpaxis, D.L., and Choli-Papadopoulou, T. (2006) Eur Biophys J 35, 657-683
  55. Indirubin-3'-Aminooxy-Acetate inhibits glycogen phosphorylase by binding at the inhibitor and the allosteric site. Broad specificities of the two sites. M.N. Kosmopoulou, D.D. Leonidas, E.D. Chrysina, G. Eisenbrand, and N.G. Oikonomakos (2005).Lett. Drug Des. Disc. 2, 377-390 Cover story
  56. The binding of IMP to Ribonuclease A. G.N. Hatzopoulos, D.D. Leonidas, R. Kardakaris, J. Kobe, and N.G. Oikonomakos (2005). FEBS J 272, 3988-4001
  57. Crystallographic studies on acyl ureas, a new class of glycogen phosphorylase inhibitors, as potential antidiabetic drugs. N.G. Oikonomakos, M.N. Kosmopoulou, E.D. Chrysina, D.D. Leonidas, I.D. Kostas, K.U. Wendt, T. Klabunde, and E. Defossa (2005).Protein Sci. 14, 1760-1771
  58. Kinetic and crystallographic studies on 2-((-D-glucopyranosyl)-5-methyl-1,3,4-oxadiazole,-benothiazole, and -benzimidazole, inhibitors of muscle glycogen phosphorylase b. Evidence for a new binding site. E.D. Chrysina, M.N. Kosmopoulou, C. Tiraidis, R. Kardakaris, N. Bischler, D.D. Leonidas, Z. Hadady, L. Somsak, T. Docsa, P. Gergely, and N.G. Oikonomakos. (2005).Protein Sci. 14, 873-888.
  59. Binding of beta-D-glucopyranosyl bismethoxyphosphoramidate to glycogen phosphorylase b: Kinetic and crystallographic studies. E.D. Chrysina, M.N. Kosmopoulou, R. Kardakaris, N. Bischler, D.D. Leonidas, T. Kannan, D. Loganathan, and N.G. Oikonomakos (2005). Bioorg. Med. Chem. 13, 765-772
  60. Ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus display multiple defects in ribosomal functions and sensitivity against erythromycin. Tsagkalia, A., Leontiadou, F., Xaplanteri, M.A., Papadopoulos, G., Kalpaxis, D.L., and Choli-Papadopoulou, T. (2005) RNA 11, 1633-1639
  61. Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice. Kontou, M., Govindasamy, L., Nam, H.J., Bryant, N., Llamas-Saiz, A.L., Foces-Foces, C., Hernando, E., Rubio, M.P., McKenna, R., Almendral, J.M., and Agbandje-McKenna, M. (2005) J. Virol. 79, 10931-10943.
  62. Estrogen receptors alpha and beta (ERalpha and ERbeta) and androgen receptor (AR) in human sperm: localization of ERbeta and AR in mitochondria of the midpiece Solakidi, S., Psarra, A.-M.G., Nikolaropoulos, S., Sekeris, C.E. (2005). Hum Reprod. 20, 3481-3487.
  63. Glucocorticoid receptor isoforms in human hepatocarcinoma HepG2 and SaOS-2 osteosarcoma cells: presence of glucocorticoid receptor alpha in mitochondria and of glucocorticoid receptor beta in nucleoli. Psarra, A.-M.G., Solakidi, S., Trougakos, I.P., Margaritis, L.H., Spyrou, G., Sekeris, C.E. (2005). Int J Biochem Cell Biol. 37, 2544-2558.
  64. Differential subcellular distribution of estrogen receptor isoforms: localization of ERalpha in the nucleoli and ERbeta in the mitochondria of human osteosarcoma SaOS-2 and hepatocarcinoma HepG2 cell lines. Solakidi, S., Psarra, A.-M.G, Sekeris, C.E. (2005) Biochim Biophys Acta. 1745, 382-392.
  65. Critical Role of Residues Defining the Ligand Binding Pocket in Hepatocyte Nuclear Factor-4. Aggelidou, E., Iordanidou, P., Tsantili, P., Papadopoulos, G., and Hadzopoulou-Cladaras, M. (2004) J. Biol. Chem. 279, 30680-30688.
 
6. Thesis:
Final Year Projects
  1. Elena Prokova Biochemical studies on eosinophil derived neurotoxin (2013) (Advisor: D.D. Leonidas)
  2. Panagiota Ligri Assessment of natural extract for hypoglycaemic activity (2013) (Advisor: D.D. Leonidas)
  3. Eirini Xemantilotou Purification and biochemical characterization of tomatinase from fusarium solani (2013) (Advisor: M. Kontou)
  4. Angelliki Angellopoulou Evaluation of antitumor activity of selective modulators of estrogen receptors (2013) (Advisors: M.N. Alexis & D.D. Leonidas)
  5. Vassiliki Kolotroni Immobilized trypsin for proteomic analyses (2013) (Advisors: G. Massolini & D.D. Leonidas)
  6. Kalliopi Pegiou Αssessment of polyphenolic extracts from Vitis Vinifera for hypoglycemic action (2013) (Advisor: D.D. Leonidas)
  7. Nikos Nikoleousakos Assessment of glycogen phosphorylase inhibitors for their drugability with computational methods (2013) (Advisor: G. Papadopoulos)
  8. Stavroula Theophanous Kinetic and crystallographic studies of glycogen phosphorylase inhibitors as potential antidiabetic agents (2012) (Advisor: D.D. Leonidas)
  9. Apostolos Gevrekis Glycogen phosphorylase inhibitors as antidiabetic agents (2012) (Advisor: D.D. Leonidas)
  10. Athina Georgiadou Kinetic studies of glucosidic analogues as inhibitors of glycogen phosphorylase b (2012) (Advisor: D.D. Leonidas).
  11. Eleni Mavridou Biochemical studies of glycogen phosphorylase for the design of hypoglycaemic drugs (2012) (Advisor: D.D. Leonidas).
  12. Zisis Koutsogiannis The binding of 5 substituted glycopyranose nucleotides to glycogen phosphorylase b (2012) (Advisor: D.D. Leonidas).
  13. Evangelia Iliana Adamou Kinetic and crystallographic studies of glycogen phosphorylase inhibitors with the goal to design new hypoglycaemic agents (2012) (Advisor: D.D. Leonidas).
  14. Maria Tsioumpekou The effect of nucleosides analogues on the ribonuclease enzymatic activity (2011) (Advisor: N. Balatsos).
  15. Georgia Dimoulou Expression studies of the subunit 7 from the CNOT deadynalation complex in leukemia (2011) (Advisor: N. Balatsos).
  16. Maria Hatzi Interacting factors with polyU sequences (2011) (Advisor: N. Balatsos).
  17. Thomas Plakas Relationship of the G2576U, C2534U, and G2576U+C2534U mutations on the23S rRNA from Staphylococcus epidermidis strains with resistance in the antibiotic linezolid. (2011) (Advisor: G. Papadopoulos).
  18. Martha Savidou Inhibition of glycogen phosphorylase as a target for the design of new hypoglycaemice agends: kinetic and crystallographic studies. (2011) (Advisor: D.D. Leonidas).
  19. Michael Kodounis Control of the transcription activity of the glucocorticoid receptor from the transcription factor Prox-1. (2011) (Advisor: A.-M. Psarra).
  20. Paschalina Pallaki (2010) Studies on the bioligcal significance of the human deadenylase CNOT7 (Advisor: N Balatsos).
  21. Savvina Moustafa (2010) Studies on the regulation of the brain sodium-urine peptide in physiological and cancer human cells (Advisor: N Balatsos).
  22. Anastasia Tomatsidou (2010) AtNOC, a new deadenylase regulated by the circadian rhythm in Arabidopsis thaliana (Advisor: N Balatsos).
  23. Diana Skoutelnik (2010) Studies on the biological role of the human deadenylase Nocturnin (Advisor: N Balatsos).
  24. Maria Maltezaki (2010) Expression studies of the poly(A) specific ribonuclease (Advisor: N Balatsos).
  25. Argiri Stamatoula (2010) Isolation and biochemical characterization of tomatinase from the fungus Fusarium solani strain FsK. (Advisor: M. Kontou).
  26. Dimitrios Pouliosς (2010) Statistical study of the degenerative lesions of the cervical spine - the effect of sleep habits as predisposing factors. (Advisor: B. Bagiatis)
  27. Evangelos Tsigaridas (2010) Denaturation of von Willebrand factor in hemostasis in 8 M urea concentration with molecular dynamics simulations. (Advisor: G. Papadopoulos)
  28. Vicky Stathi (2010) Denaturation of the A, Α2, Α3 subunits of the von Wilebrand factor with constant power exercise during molecular dynamics simulations (Advisor: G. Papadopoulos).
  29. Vassilios-Chrisobalantis Palios (2010) Relationship of the U2504A and C2534U mutations on the 23S rRNA from Staphylococcus epidermidis with resistance to the antibiotic linezolid (Advisor: G. Papadopoulos)
  30. Agelliki Deligianni (2010) The role of PAI-1 in the occurrence of myocardial infarction in young people (Advisor: M. Kontou)
  31. Stefanos Fakiolas (2010) Study of gene polymorphisms of the receptor of the low density lipoprotein (LDL-R) in familial hypercholesterolemia (Advisor: M. Kontou)
  32. Stelios Karoulias (2009) Biochemical and molecular characterization of the tomatinase from fungus Fusarium solani strain FsK (Advisor: M. Kontou)
  33. Anastasia Kansadi (2009) Proteomic analysis of the wild and mutant strain of Pseudomonas entomophila (Advisor: M. Kontou)
  34. Eva Marakaki (2009) Overexpression, purification and structural studies of the carboxy-terminal domain 348-826, with S641àE replacement, of the hypoxia induced 1α (HIF1α) factor (Advisor: M. Kontou)
  35. Ekaterini Karagiorgou (2009) Overexpression, purification, and structural studies of the recombinant poly(A) – specific ribonuclease (PARN). (Advisor: D.D. Leonidas)
  36. Stavros Papatzelos (2009) Structural comparison of protein complexes and isolated polypeptide chains after equalization with molecular dynamics simulations (Advisor: G. Papadopoulos)
  37. Dimitra Leventi (2009) The effect of synthetic noucleotides on poly(A) – specific ribonuclease (PARN). (Advisor: N. Balatsos)
  38. Βasiliki Chatzigeorgiou (2009) Inhibition of the degradation of poly (A) tail from synthetic analogues and nucleoside substrates ((Advisor: N. Balatsos)
  39. Βasiliki Zervakaki (2009) The effect of nucleoside analogues on the enzymatic activity of the bovine pancreatic ribonuclease (RNase A) (Advisor: N. Balatsos)
  40. Vassiliki Tsirkoni (2008) Structural and kinetic studies of the RNase A in complex with U5P and UDP. (Advisor: M. Kontou)
  41. Maria Tziastoudi (2008) Installation of the molecular modeling software package MODELLER and applications on the von Willebrand factor. (Advisor: G. Papadopoulos)
  42. Panagiotis Maragozidis (2008) The effect of noucleotide analogues on poly(A) specific ribonuclease PARN. (Advisor: N. Balatsos)
  43. Theodosia Dimopoulou (2008) Silencing of the poly(A) specific ribonuclease PARN with the use of double-clone RNAs in human cancer cells (Advisor: N. Balatsos)
  44. Εrgina Vourkou (2008) Overexpression, purification and structural study of the carboxyterminal domain 348-826 of the hypoxia induced 1α (HIF1α) factor (Advisor: M. Konotu)
  45. Dimitrios Anastasakis (2007) Overexpression, purification and studies on the enzymatic activity of poly(A) specific ribonuclease PARN. (Advisor: N. Balatsos).
  46. Μaria Papachristou (2005) Study of the cancer antigen Her/neu (Advisor: M. Kontou)
M.Sc. Thesis
  1. Georgios Stravodimos (2013) Assessment of polyphenolic extracts from byproducts of the pomegranate and grape industrial process for hypoglycaemic activity by biochemical methods (Advisor: D.D. Leonidas)
  2. Chrysogelloy Eleni (2013) Inhibitors of glycogen phosphorylase as potential antidiabetic drugs (Advisor: D.D. Leonidas)
  3. Evridiki Mpoulimari (2013) Assessment of the hypoglycaemic activity of polyphenolic extracts from byproducts of the pomegranate industrial process for hypoglycaemic activity by biochemical methods (Advisor: D.D. Leonidas)
  4. Aikaterini Kotsona (2012) Assessment of natural compounds as inhibitors of glycogen phosphorylase for the design of new hypoglycemic agents (Advisor: D.D. Leonidas)
  5. Stamatoula Argiri (2012) Glycogen phosphorylase inhibitors as potential antidiabetic drugs (Advisor: D.D. Leonidas)
  6. Vassiliki Pepa (2012) Crystal Structure of two variants of the Sclerotium rolfsii lectin (Advisor: D.D. Leonidas)
  7. George Oikonomakos (2012) Kinetic and crystallographic studies on the binding of a new glucopyranosyl analogue to glycogen phosphorylase b (Advisor: D.D. Leonidas)
  8. Stilianos-Zafeirios Karoulias (2012) Assessment of Triazole pyrimidine nucleosides as inhibitors of Ribonuclease A (Advisor: N. Balatsos)
  9. Αnastassia Kantsadi (2011) Glycogen phosphorylase inhibitors as potential hypoglycaemic agents. (Advisor: D.D. Leonidas)
  10. Εrgina Vourkou (2011) Crystallization and structural studies of the xanthine oxidase (Advisor: D.D. Leonidas)
  11. Μaria Tziastoudi (2011) Parametrization of the antibiotic linezolide for Charmm force field. Simulation of the 50S ribosome subunit from E.coli in complex with linezolide (Advisor: D.D. Leonidas)
  12. Αthina Totomi (2010) Study of the deadenylase biological role in patients with lung cancer (in progress) (Advisor: N. Balatsos)
  13. Dimitra Xhatzileontiadou (2010) Biological role of the deadenylases CNOT6 and CNOT8 (in progress) (Advisor: N. Balatsos).
  14. Cryssoula Gakou (2010) Cloning, overexression and study of the Α1 and Α2 subunits of the blood coagulation factor von Willebrand (Advisor: D.D. Leonidas)
  15. Christina Siouta (2010) Study of the A1 and Α2 subunit interaction from the blood coagulation factor von Willebrand with site-directed mutagenesis (Advisor: D.D. Leonidas)
  16. Georgia Batra (2010) Purifcation and structural studies of phosphorylase from solanum tuberosum. (Advisor: D.D. Leonidas)
  17. Αthanasios Kyritsis (2010) Studies on the biological role of the poly(A) specific ribonuclease PARN (Advisor: N. Balatsos).
  18. Μaria Labrou (2010) Studies on the role of subunit 7 of the CCR4-NOT complex (Advisor: N. Balatsos).
  19. Μaria Karageli (2010) Expression study of the poly(A) specific ribonuclease PARN in patients with leukemia (Advisor: N. Balatsos).
  20. Κonstantina Papaspyrou (2010) Expression studye of deadenylases in patients with leukemia (Advisor: N. Balatsos)
  21. Christina Spanou (2009) The binding of 5’ phosphate guanosine to eosinophil derived neurotoxin: kinetic and crystallographic studies (Advisor: D.D. Leonidas)
Ph.D. Theses
  1. Zoi Karoulia Effect of platelets in the individual reactions of haemostasis (in progress) (Advisor: D.D. Leonidas)
  2. Stelios Floros Study the behavior of proteins in alternating electric field in molecular dynamics simulations (in progress) (Co-advisor: G. Papadopoulos).
 
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 Professor Demetres D. Leonidas
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Tel. 2410-565278
Fax. 2410-565290
Email. This e-mail address is being protected from spambots. You need JavaScript enabled to view it
 
Assistant Professor M. Kontou
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Τel. 2410-565281
Fax. 2410-565290
Email. This e-mail address is being protected from spambots. You need JavaScript enabled to view it
 
Assistant Professor N. Balatsos
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Τel. 2410-565261
Fax. 2410-565290
Email. This e-mail address is being protected from spambots. You need JavaScript enabled to view it
 
Assistant Professor G. Papadopoulos
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Τel. 2410-565249
Fax. 2410-565290
Email. This e-mail address is being protected from spambots. You need JavaScript enabled to view it
 
Assistant Professor Α.-Μ. Psarra
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Τel. 2410-565221
Fax. 2410-565290
 
 
Assistant Professor V. Skamnaki
Department of Biochemistry and Biotechnology
University of Thessaly
Ploutonos 26
41221 Larissa
Τel. 2410-565267
Fax. 2410-565290
Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
 

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